I would make sure to include questions that revolve around growth, size, and reproduction in order to hammer home the importance of how precious the resource is.
(1) During what month do musky typically spawn? What water temperature range do they spawn in? (2) What kind of lake areas do musky spawn in? (3) In general, what is the top forage item for musky? The second forage item? (4) In general, what percentage of the musky diet is represented by these top two forage items? (5) On average, how many years does it take a musky to reach 30, 40, and 50 inches in length? (6) What are the benefits of increasing the minimum size limit for harvesting a legal musky? (7) Most musky anglers think that holding a musky vertically and/or laying a fish down on a carpeted boat surface are dangerous for the musky. Explain why.
There are countless questions you could ask. The ones above are the first ones that came to mind when I read your question.
Have a mixture of question formats, too. Have some short answer, some true/false, some fill-in-the-blank, some multiple choice, etc. Short answer are the best since they require the most knowledge. It also reveals why they're wrong since they have to write down the answer. That kind of information is just as valuable.